Introduction
STC-96A PLUS is a real-time PCR system built for fast and accurate gene expression analysis, genetic variation studies and High-Resolution Melting Curve (HRM) applications. As a quantitative PCR machine, it combines 96-well capacity, dual-block flexibility, precise thermal control and multi-channel fluorescence detection to support both routine PCR testing and advanced molecular research.
For laboratories comparing PCR machine brands or upgrading an existing thermal cycler PCR machine, STC-96A PLUS offers a practical balance of performance, flexibility and workflow efficiency. It can be used as standalone real-time PCR equipment or integrated into a broader PCR automation system with Bioperfectus solutions for sample preparation, PCR setup and result interpretation.
Figure 1. Partial enlarged plot of STC 96A Plus's temperature curve shows temperature uniformity cross 96 wells within 0.05℃
Figure 2. STC-96A Plus multi-tasking software interface (analysis screen)
Features
- Exclusive efficiency and flexibility
Innovative dual 48/48 reaction blocks allow this real-time PCR machine to be controlled separately for two different assays running simultaneously on one instrument
- Precise temperature control
Well-to-well uniformity with an accuracy of 0.1℃ for reliable performance of the PCR instrument
- Fast and accurate results
A super-fast heating/cooling rate of up to 4.0℃/sec makes it a rapid PCR machine for efficient testing
- No crosstalk calibration needed
Professional optical filter combinations eliminate crosstalk between FAM, HEX, ROX and CY5 channels
With long-lasting, maintenance-free LEDs and an 18-month warranty, it delivers dependable performance for daily real-time PCR testing.
Parameters
|
Model
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STC-96A PLUS
|
|
Sample capacity
|
96 wells
|
|
Parallel running mode
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Dual Reaction blocks/running 2 tests independently
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|
Reaction volumes
|
15-100 µL
|
|
Consumable
|
0.2ml PCR tubes, 8-tube strips, 96-well plates
|
|
Run time
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<50 mins
|
|
Temperature range
|
4-99 ℃
|
|
Max ramp rate
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4.0℃/sec
|
|
Temperature accuracy and uniformity
|
0.1℃
|
|
Heating and cooling method
|
Peltier
|
|
Temperature control mode
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Tube control/block control
|
|
Excitation Source
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LED (maintenance-free)
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|
Detector
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High sensitivity photoelectric sensor
|
|
Sensitivity
|
1 copy
|
|
Reproducibility
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CV<0.5%
|
|
Dynamic range
|
100 - 1010 copies/well detectable
|
|
Excitation range
|
CH1-CH4: 470-630 nm; CH5/CH6: customer-made
|
|
Detection range
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CH1-CH4: 510-665 nm; CH5/CH6: customer-made
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|
Dyes and probes
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FAM™/SYBR Green®; VIC®/HEX™/JOE™/TET™; ROX™/Texas Red®; CY5™
|
|
Dimensions (W×D×H)
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38cm×52cm×25cm
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|
Weight
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18 kg (40 lbs)
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|
Computer
|
Pentium PC with Windows XP, Vista or Windows 7/8
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|
Communication
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RS232, USB
|
|
Power Supply
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230V, 50HZ
|
|
Power Consumption
|
850VA
|
Applications
The STC-96A PLUS real-time PCR system is designed for a wide range of qPCR and molecular diagnostics applications, including:
- Gene expression analysis
- Genetic variation analysis
- High-Resolution Melting Curve (HRM) applications
- Research and clinical laboratory qPCR testing
Combined with Bioperfectus real-time PCR kits and molecular diagnostics solutions, the system helps laboratories build efficient workflows for respiratory infections, HPV and cervical cancer screening, sexually transmitted infections, vector-borne diseases and other infectious disease testing needs.
Certificates
CE, FDA-Listed
