Vibrio Cholerae Real Time PCR Kit

The Vibrio Cholerae Real Time PCR Kit is an in vitro diagnostic assay for the qualitative detection of Vibrio cholerae DNA in human stool specimens. The multiplex PCR test detects Vibrio cholerae and differentiates the O1 and O139 serogroups to support laboratory cholera diagnosis.

Vibrio cholerae is a Gram-negative bacterium that causes cholera, a severe infection of the small intestine. Transmitted through contaminated food or water, it releases cholera toxin, leading to rapid dehydration and critical electrolyte loss through acute diarrhea and vomiting. Cholera remains a major global public health threat, prone to sudden outbreaks and pandemics.

Designed for gastrointestinal infectious disease laboratories, the assay combines a 5-copy-per-reaction limit of detection with an internal control. A 200 μL sample input is specified in the product parameters, supporting the detection of Vibrio cholerae by PCR within a standardized laboratory workflow.

• Multiplex cholera detection: Detects Vibrio cholerae and differentiates O1 and O139 serogroups.

• Human stool specimens: Built for V. cholerae stool test workflows.

• Reliable: 5 copies/reaction (10³ copies/mL) with internal control

• Efficient testing: Processes 96 samples in 62 minutes, with automated result interpretation available on the STC real-time PCR system.

The kit is intended for professional cholera testing of human stool. Applications include:

• Laboratory cholera diagnosis support: Qualitative detection of Vibrio cholerae DNA when cholera is considered in patients with compatible clinical findings.
• O1/O139 differentiation: Multiplex testing that distinguishes the two stated serogroups while detecting V. cholerae DNA.
• Gastrointestinal molecular testing: Use in clinical laboratories processing human stool specimens on compatible real-time PCR systems.

CE